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Analysis & Separations

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Synthesis and trafficking of the tonoplast potassium channel AtTPK1

Poster

Published: August 20, 2009

Synthesis and trafficking of the tonoplast potassium channel AtTPK1 content piece image

Sorting signals of tonoplast proteins and the pathway they follow through the endomembrane system are still poorly characterized. Using Brefeldin A (an inhibitor of the Golgi-mediated traffic) we showed that a Golgi-dependent pathway for tonoplast delivery may exist. Then we generated various chimeric TPK1-TPK4 fusions, exchanging various domains. We observed that the cytosolic, C-terminal domain of TPK1 is sufficient to re-direct the plasma membrane TPK4 to the tonoplast.