AB SCIEX Expands Eksigent Chip Column Family for Nano and Micro LC Proteomics Analysis
The extended offering adds higher flow micro cHiPLC columns for peptide separation at increased throughput levels, as well as new phases for the analysis of intact proteins, phosphopeptides and glycans. All cHiPLC-nanoflex columns are designed and microfabricated to be robust and highly reproducible, providing superior-quality separations that are easy to obtain by users at all experience levels.
The first two of the new chips are nano LC columns – C4 for the analysis of intact proteins and graphitized carbon for phosphopeptides and glycans. The other three new chips, including 200 µm i.d. C18-CL micro cHiPLC columns in 15 cm and a new shorter 5 cm length, are run at 1.0 to 3.5 µL per-minute flow rates, which reduce gradient delay times compared to nano flow HPLC, providing higher sample throughput and improved chromatography. The new micro cHiPLC columns deliver robust performance as well as increased speed and throughput for applications, such as quantitative biomarker validation.
When tested against the standard 75 µm i.d., the new 200 µm chips five times more protein identifications. This was achieved by loading five times more sample on the new chip, with a yeast digest, resulting in three times the number of distinct peptide identifications.
“Chromatography on the nano and micro scale has traditionally suffered from reproducibility and robustness limitations, and our cHiPLC columns deliver significant improvements.”- said Remco van Soest, Director of Eksigent HPLC Product Development for AB SCIEX.
“This is part of our effort to develop and introduce new cHiPLC columns on an ongoing basis to meet the needs of our proteomics customers.”
Nanoflow liquid chromatography coupled with nanoelectrospray MS is the method of choice for protein and peptide analysis. The cHiPLC-nanoflex System is a docking station for up to three column chips. It can be added to any of Eksigent’s nanoLC systems, which can be interfaced to many mass spectrometers, including AB SCIEX TripleTOF™ 5600 for biomarker discovery and the AB SCIEX QTRAP® 5500 for verification and validation. Eksigent’s proprietary EksPortTM microconnector system ensures reliable and consistent connections to facilitate column changes.
The new nanoLC columns as well as trap columns for all phases are available for immediate shipment. The micro cHiPLC columns, as well as a new nanoLC HALO* HILIC phase will be available in October of this year.