AnaSpec Introduces a Line of Reagents and Kits for Study of Protein Phosphorylation
Product News Jun 06, 2006
AnaSpec has presented the comprehensive collections of peptides and related reagents to assist in investigating the functions of protein kinases and phosphatases in biological systems.
Utilizing optimized Fmoc methodologies and orthogonal protection strategy for multiple phosphorylated peptides, AnaSpec's chemists routinely synthesize mono-, di-, tri- or multiple phosphoamino acids sequences at its facilities in San Jose, CA.
Over 280 protein phosphorylation-related peptides, including substrates for Calcium/Calmodulin-dependent protein kinases; cAMP related kinases; Cyclin-dependent kinase 5 (CDK5); Kemptide; MAPK (including EGFR) and PKC, Protein Tyrosine Kinase (PTK) and more.
These substrates include both phosphorylated and non-phosphorylated; fluorophore (green or red) or biotin-labeled kinase; and, phosphatase substrates.
Contains 6 individually-packed phosphopeptides at 1 ug each. These phosphopeptides can be used for the characterization of affinity purified phosphorylated peptides in liquid chromatography/mass spectrometry.
FAM-, TAMRA-, or Biotin-Labeled: Designed to facilitate the characterization of an appropriate peptide substrate for developing a particular Ser/Thr kinase or Tyr protein kinase assay, each kit contains a series of FAM, TAMRA, and/or Biotin-labeled protein kinase substrates.
In addition to colorimetric pNPP-based assay kits, AnaSpec also provides a series of FDP and MFP-based fluorimetric assay kits for enhanced measurement of phosphatase activity.
These fluorimetric assays are 50-100X more sensitive than pNPP; have a wider dynamic range; and can be monitored using the popular green fluorescence filter (Ex/Em=490/520 nm).