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Berthold Launches TriStar² Microplate Reader

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Berthold Technologies presents the 2nd generation of its multi-label TriStar² microplate reader.

TriStar² employs the new patent pending optical concept ALL-4-ONE, for the first time enabling luminescence, fluorescence and absorbance measurements with highest respective sensitivity as known from dedicated instruments. TriStar² is also capable of reading FRET, BRET and BRET².

In comparison to its predecessor the optics has been further improved and an improvement in detection limits in fluorescence has been achieved
• Less than 6 amol ATP per well
• Less than 0.3 fmol Fluorescein per well

The instrument is characterized by an universal detector with extremely low noise for fluorescence and luminescence measurements and it is equipped with a photo diode for absorbance readings in addition.

Furthermore optical filters can be used for luminescence measurements enabling BRET (e.g. functional assays for GPCR research) and multi-coloured luciferase reporter gene applications.

TriStar² can be fitted with up to 3 reagent injector - based on the proven and most accurate JET technology - and temperature control for the microplate compartment.

Two of the injectors have their tips in reading position enabling the measurement of extremely fast reactions.

New to the instrument is the reagent compartment placed at the front where the reagents are easily accessible and where they can be kept cooled by the addition of crushed ice when needed.

For increased sample throughput TriStar² can integrated into lab automation systems.

Special reagent mounts provide a safe fixation of small reagent vials und ensure complete consumption of the reagents through inclination of the vial.

Thus, TriStar² microplate reader offers a multitude of possibilities and a wide range of applications. Measurements of enzyme activities, phagocytosis, Calcium flux, cell viability, apoptosis, immuno assays, protein and DNA concentrations and protein-protein interactions are only a few of the many uses of TriStar².

The unit is operated via the proven and intuitive ICE software. Single and multiple endpoint readings are possible as well as kinetic and scanning measurements.

The data are displayed in numerical and graphical formats and may be exported into EXCEL or printed.