Biotium Expands Cheetah™ Taq Portfolio With New Formulations for Fast Cycling PCR and Proximity Extension Assays
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Biotium, proudly expands its trusted Cheetah™ Taq DNA Polymerase line with two new formulations: Cheetah™ FLEX HotStart Taq DNA Polymerase and Cheetah™ PEA-PLEX HotStart Taq DNA Polymerase.
These optimized versions of Biotium’s chemically modified, antibody-free hot-start Taq offer unmatched activation speed and performance for modern workflows, including fast-cycling qPCR, proximity extension assays (PEA), and NGS-based high-throughput PEA for protein detection. While Cheetah™ Taq has been a reliable choice for reducing non-specific amplification in PCR, these newly tailored formulations bring greater flexibility and targeted performance to meet the evolving needs of researchers and suppliers of high-throughput commercial assays.
Cheetah™ FLEX HotStart Taq: Fast-Cycling & PEA Versatility
Cheetah™ FLEX is engineered for speed and adaptability across a range of applications. With a rapid 2-minute activation at 95°C, FLEX delivers up to 10x faster activation than AmpliTaq Gold® or HotStartTaq®, with none of the drawbacks of antibody-based hot-start enzymes. The antibody-free formulation eliminates animal DNA contamination and provides enhanced long-term stability. Cheetah™ FLEX is validated for fast-cycling PCR and qPCR-based PEA, making it a go-to choice for both routine and advanced workflows. After heat activation, Cheetah™ Taq has the same functional properties and fidelity as native Taq polymerase.
Cheetah™ PEA-PLEX HotStart Taq: Optimized for NGS-Based High-Throughput PEA
Cheetah™ PEA-PLEX is a unique formulation of Cheetah™ Taq developed specifically for high-throughput, NGS-based PEA assay for protein detection. Third-party validated in high-throughput PEA, Cheetah™ PEA-PLEX is also compatible with fast-cycling qPCR.
Key Features of Cheetah™ HotStart Taq DNA Polymerase:
- Novel chemical hot-start mechanism: No antibodies, no activation lag
- Rapid 2-minute activation at 95°C
- Antibody-free, low-DNA enzyme for contamination-free results
- Superior long-term stability than traditional chemically modified Taq
- Versatile use for fast-cycling qPCR and PEA
- Cheetah™ PEA-PLEX developed and validated for NGS-based PEA assays
- Available in ready-to-use kits and customizable bulk formats
Cheetah™ Taq requires only 2 minutes of heat activation for full recovery of activity. Comparison of hot-start recovery of polymerase activity for Cheetah™ Taq and AmpliTaq Gold® following incubation at 95°C in 50 mM pH 8.0 Tris.
