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Differential Ion Mobility Interface Boosts Proteomics Workflow Performance

Differential Ion Mobility Interface Boosts Proteomics Workflow Performance content piece image
Thermo Scientific Orbitrap Exploris 480 mass spectrometer
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Proteomic scientists can now take advantage of an advanced differential ion mobility interface that seamlessly integrates with Thermo Fisher Scientific’s range of new-generation mass spectrometers for workflow efficiency and enhanced data quality. The interface is ideal for experiments ranging from the discovery of and routine testing for potential disease biomarkers, to the identification of new therapeutic targets.

When combined with a new-generation mass spectrometer like the new Thermo Scientific Orbitrap Exploris 480 mass spectrometer or the new Thermo Scientific Orbitrap Eclipse Tribrid Mass Spectrometer, the Thermo Scientific FAIMS Pro interface is designed to increase the breadth and depth of proteins scientists can identify and quantify, while also reducing time-consuming sample preparation steps. Furthermore, the FAIMS Pro interface improves the signal-to-noise ratio and limits of quantitation for routine testing when coupled with a Thermo Scientific triple stage quadrupole mass spectrometer.

Thermo Fisher Scientific is showcasing the FAIMS Pro interface during the 67th American Society for Mass Spectrometry (ASMS) Conference on Mass Spectrometry and Allied Topics, being held June 2–6, in the International Ballroom ABCD at the Omni CNN Center Hotel, Atlanta, Georgia.

Josh Coon, Thomas and Margaret Pyle chair at the Morgridge Institute for Research, professor of Biomolecular Chemistry and Chemistry at the University of Wisconsin, Madison, and director of NIH National Center for Quantitative Biology of Complex Systems, said, "We have been extremely impressed with the performance of the FAIMS Pro interface for shotgun proteomics. The FAIMS Pro interface enables considerably more sample depth per unit time and could eliminate the need for pre-fractionation of peptides for most applications.”

“Scientists increasingly demand improved protein coverage during proteomic experiments; increased dynamic range to comprehensively characterize and validate proteins and their post-translational modifications; and more accurate quantitation of hundreds to thousands of proteins in a single run in shorter analysis times,” said Iain Mylchreest, vice president, R&D, for Thermo Fisher’s analytical instruments business. “Building on last year’s enhancements to the industry-leading Thermo Scientific Tribrid platform, the FAIMS Pro interface provides new analytical performance that is expected to reduce sample and spectral complexity, improve selectivity and increase coverage of the proteome.”

The FAIMS Pro interface is designed to improve analytical performance compared to existing interface options by:
• Providing faster, higher-quality results through its advanced design, further enhancing instrument selectivity and detection limits through gas phase fractionation and reduced matrix interference;
• Leveraging menu-driven software to design methods using pre-configured parameters, increasing productivity and simplifying use;
• Reducing sample fractionation steps, which can help save time, long-term costs and maintenance through increased productivity; and
• Achieving high data quality on sample-limited studies through an interface exclusively designed to improve nano, capillary and microflow applications.

When the interface is combined with the new, intelligence-driven Thermo Scientific Orbitrap Exploris 480 mass spectrometer, an instrument from the Thermo Scientific Triple Quadrupole mass spectrometry range or the Thermo Scientific Orbitrap Tribrid mass spectrometry range, laboratories have access to a comprehensive solution that improves selectivity and ensures increased productivity across a range of proteomics workflows.