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Efficient QC Method for Determination of Crotamiton, a Treatment for Scabies and Skin Pruritus


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Application Brief 138 describes a convenient reversed-phase high-performance liquid chromatography (RP-HPLC) method using a Thermo Scientific Acclaim C30 column with a simple acetonitrile/water mobile phase. The Acclaim™ C30 column is designed to provide high shape selectivity for separation of hydrophobic, structurally related isomers and unique selectivity complementary to other RP columns (e.g., C18).

Although another method for analysis of cis and trans isomers of Crotamiton by RP-HPLC has been reported, it requires a three-component mobile phase (acetonitrile/phosphate buffer containing 1% triethylamine) to achieve satisfactory results.

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