Fast Determination of Anthocyanins in Fruit Juice

This method is detailed in Application Note 264: Fast Determination of Anthocyanins in Pomegranate Juice and demonstrates how the separation of anthocyanin compounds in various fruit juices can be achieved in less than 5 min. The method uses a high-resolution, silica-based, Acclaim® RSLC C18 column and absorbance detection at a visible wavelength of 540 nm to separate and detect these compounds.

Six of the anthocyanidins that occur most commonly in nature are pelargonidin, cyanidin, peonidin, delphinidin, petunidin, and malvidin. Due to their strong antioxidant properties, anthocyanins are of considerable interest to the scientific community and consumer market. Increased understanding of their health benefits has led to a growing interest in determining anthocyanins in foods, nutraceuticals and natural products.