Fast Plasma Sample Cleanup with New Phree™ Phospholipid Removal Plates
Product News May 22, 2012
In one step, Phree removes both proteins and phospholipids and delivers the prepared plasma to a collection plate. The high-capacity Phree sorbent can process up to 400 µL per well and removes 99.0 to 100 percent of lysophosphatidyl and phosphatidyl cholines, which are not removed when a simple protein precipitation is performed.
Successful chromatographic analysis of plasma samples requires the removal of both proteins and phospholipids, which can clog HPLC/UHPLC columns. Phospholipids can also cause ion suppression and over time will reduce sensitivity due to buildup on the mass spec source. Phree removes both proteins and phospholipids in one step, eliminating time-consuming method development. The plate format enables the simultaneous processing of 96 samples, for significant time savings.
Nina Khoshaba of WIL Research was an early-access user of the new Phree product. She comments, “We have demonstrated that Phree Phospholipid Removal Plates resulted in a decrease of more than 98 percent in the peak area of the six phospholipids we monitored in rat plasma compared to traditional protein crash. We highly recommend the use of these plates for high-throughput LC-MS/MS bioanalytical sample preparation.”
“Whether your target analyte is an acid, base, or neutral , Phree cleans up plasma in one fast step,” explained Erica Pike, brand manager for Phenomenex. “Phree Phospholipid Removal Plates provide high recoveries of acids, bases and neutrals without the need for method development.”