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HPLC Assay of Gentamicin Sulfate Congeners in Just 15 Minutes

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Thermo Fisher Scientific has announced a direct-detection reversed-phase high-performance liquid chromatography (HPLC) chromatographic assay method that separates the congeners in gentamicin sulfate and gentamicin sulfate-containing ointments, solutions, and creams in just 15 minutes.

Application Note 1005: Gentamicin Sulfate Assay by HPLC with Charged Aerosol Detection demonstrates that this approach is faster than previously published methods, with excellent separation between the gentamicin congeners and resolution of many impurities that can be present in fermentation-manufactured products.

This method uses a silica-based polar-embedded column that provides enhanced hydrolytic stability from pH 1.5-10 and allows resolution of a wide variety of polar and nonpolar analytes.

Use of this column allows a reduced concentration of trifluoroacetic acid, thus improving method ruggedness. Use of charged aerosol detection eliminates the need for derivatization.

With three sample matrixes analyzed, the quantification of gentamicin base had good recoveries and was consistent with the label claim of these products, both indicators of an accurate assay.