We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Linkam Scientific Instruments Launch CMS196M for Enhanced Cryo-Correlative Microscopy
Product News

Linkam Scientific Instruments Launch CMS196M for Enhanced Cryo-Correlative Microscopy

Linkam Scientific Instruments Launch CMS196M for Enhanced Cryo-Correlative Microscopy
Product News

Linkam Scientific Instruments Launch CMS196M for Enhanced Cryo-Correlative Microscopy

Credit: Linkam

Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "Linkam Scientific Instruments Launch CMS196M for Enhanced Cryo-Correlative Microscopy "

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Linkam has been developing cryo stages for correlative microscopy for many years and continues to be at the forefront of cryo-correlative microscopy with the latest update of their LINK software for the CMS196M, providing improved imaging capabilities and the new liquid nitrogen auto-fill system providing longer run times.

Cryo-correlative microscopy has become an established technique in recent years. It brings together the strengths of both electron and light (fluorescence) microscopy. Electron microscopy (EM) provides structural information at very high resolution. However, it can give only restricted insight into biological and chemical processes due to limitations in staining and sample preparation processes. On the other hand, fluorescence microscopy is an extremely sensitive technique to detect biological, chemical and genetic processes as well as events inside living cells.

Cryo-CLEM brings it all together: combining the individual advantages from both fluorescence and EM by imaging the same sample location with both techniques and superimposing the complementary information.

For biological samples to be compatible with the vacuum conditions found in EM and preserve the structural detail, samples are embedded in vitrified “glass like” ice and need to be kept below -140 °C. Any contact with moisture contained in the air has to be avoided since ice crystals would form immediately and contaminate the sample. Under cryo conditions, the fluorescence signals providing structural detail is preserved and photo bleaching is significantly reduced.

LINK software for the CMS196 provides complete control and monitoring of the system as well as fully-automated tiled image capture. The integrated, encoded, high resolution motorised XY stage gives the ability to capture an image of the entire EM grid at full resolution. Shown below, the system produces a single image of the EM grid that can then be used to navigate the sample and save co-ordinates of areas of interest making it easy to image the same area when the sample is transferred to the EM. In addition, LINK can now be supplied with an extremely cost effective, high sensitivity monochrome camera ideal for low light fluorescence imaging. 

This article has been republished from materials provided by Linkham. Note: material may have been edited for length and content. For further information, please contact the cited source.

Advertisement