New HPLC Method for Analysis of Zanthoxylum Nitidum (Roxb.) DC
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Thermo Fisher Scientific has announced an efficient and comprehensive high-performance liquid chromatography (HPLC) method for the quality control analysis of Zanthoxylum nitidum (Roxb.) DC and related products.
Application Note 1008: Determination of Nitidine Chloride, Toddalolactone, and Chelerythrine Chloride by HPLC describes the use of HPLC to rapidly separate the three main active components of Zanthoxylum nitidum (Roxb.) DC: nitidine chloride, chelerythrine chloride, and toddalolactone.
This approach is superior to both the Pharmacopeia of the People’s Republic of China (PPRC) method that measures the active components of Zanthoxylum nitidum (Roxb.) DC using two different methods-HPLC and thin-layer chromatography-and a published HPLC method that requires a long separation time.
In this new method, the determination is performed an HPLC system controlled by function-rich chromatography software.
A sulfonamide-embedded reversed-phase LC column delivers excellent peak shape for nitidine chloride and chelerythrine chloride, using an ammonium acetate buffer/acetonitrile mobile phase without triethylamine.
This application note and many others can be found at www.thermoscientific.com/dionex under the Documents tab.