New Method Speeds and Improves Glycoprotein/Glycopeptide Isolation
Product News Aug 29, 2012
Thermo Fisher Scientific has announced a new method that can automatically extract glycoproteins from samples, and glycopeptides from tryptic digests, thus enabling faster isolation, better sample recovery, high peak efficiency, and high-throughput capability.
Application Note (AN) 1014: Concanavalin A Column for Analysis of Glycoproteins and Their Tryptic Glycopeptides demonstrates that this approach enhances efficiency by leveraging innovative products.
For example, this new method uses a high-performance liquid chromatography (HPLC)-compatible Concanavalin A (Con A) column that is built on a monolithic support and offers advantages over other Con A columns.
AN 1014 also shows that a fully biocompatible analytical LC system can automate the entire off-line process, from sample enrichment and fraction collection to the reanalysis of collected sample by peptide mapping with both ultraviolet and mass spectrometry detections.
Monitoring oxonium ions with a single quadrupole mass spectrometer is a selective, sensitive, and reliable method that confirms the identity of the glycopeptides captured by the Con A column.