We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement

New Perfinity Workstation Automates Protein Sample Preparation

Listen with
Speechify
0:00
Register for free to listen to this article
Thank you. Listen to this article using the player above.

Want to listen to this article for FREE?

Complete the form below to unlock access to ALL audio articles.

Read time: Less than a minute
The Perfinity Workstation uses utilizes Shimadzu hardware components in conjunction with five Perfinity Optimized columns that each perform a step of the mass-spec sample-preparation process — affinity selection, buffer exchange, digestion, desalting and reverse-phase separation. The automated integration of these steps enables users to start with serum and have peptides ready for LC/MS analysis in 10 minutes. The automated process removes much of the equipment and labor associated with mass-spec analyses of proteins.
 
The Perfinity Workstation combines the selectivity of antibodies with the resolving power of chromatography. Buffer exchange and desalting are performed on-line. The reduction in sample-processing time enables users to screen a variety of conditions rapidly.
 
The major advantage of the Perfinity Workstation’s sample-preparation approach is that users are not required to immobilize antibodies. Instead, scientists can add antibodies directly to the sample. By removing the immobilization process, users have eliminated a potential source of error, making LC/MS analysis with Perfinity Workstation more accurate and reliable.
 
This platform is customizable across several application areas, including assay development, protein purification, drug development and biomarker discovery. For example, methods built into the workstation automate core steps of assay development, limiting the amount of protocol optimization required.