Novel Bioaffinity-MS Tandem System Developed – Combining sam5™ SAW Biosensor with ESI Mass Spec
Want to listen to this article for FREE?
Complete the form below to unlock access to ALL audio articles.
Read time: 1 minute
Researchers at the University of Konstanz, have for the first time successfully developed a novel on-line bioaffinity-electrospray ionisation (ESI) mass spectrometry approach, which enables the simultaneous label-free detection, identification and quantification of protein–ligand interactions. Combining the sam5™ surface acoustic wave (SAW) biosensor from SAW Instruments directly with ESI-MS has enabled the direct connection of protein-ligand KD analysis with their subsequent quantification and structural characterisation by mass spec.
This new tandem SAW-ESI-MS system has been developed in the laboratory of Professor Michael Przybylski at the University of Konstanz and recently published (Dragusanu et al, 2010)1. Biosensors have previously only ever been connected to mass spec indirectly in a MALDI-TOF format where crystallised sample spots are analysed. The new system directly connects liquid flow from the SAW biosensor directly into the ESI-MS via a standard desalting interface – an approach impossible with other available biosensors. This novel set-up enables direct quantitative determinations of protein-ligand complexes by SAW-yielded dissociation constants (KD) from low nanomolar to sub-micromolar sample concentrations.
The Przybylski team observed the key advantages of SAW in comparison to classical immuno-analytical bioaffinity techniques to be the direct and rapid determination of association/dissociation constants from small and dilute sample amounts, without the need for labelling or recalibration for buffer changes. Furthermore, the sam5 biosensor is able to make detection measurements in complex biological samples e.g. crude brain samples, blood or cell lysates.
The new SAW-ESI-MS technique has been successfully applied by the Przybylski group to a number of clinically relevant applications for biomarker discovery, analysis and epitope mapping. These include human ß-amyloid peptides (Alzheimer’s Disease), Substance P peptide-calmodulin complex, tyrosine-nitrated peptides (neurotoxic events) and human ß-synuclein (Parkinson’s Disease). Indeed, no details on affinity binding and KD determination of the anti-a-synuclein-human a-synuclein complex have previously been reported.
“We are confident that the new on-line SAW-ESI-MS approach utilizing the sam5™ biosensor will develop into an efficient and sensitive tool for the label-free detection, identification and quantification of biopolymer-ligand interactions as diverse as antigen-antibody and lectin-carbohydrate complexes,” said Professor Michael Przybylski. “Certainly SAW technology is now becoming increasingly popular for the study of biomacromolecular interactions due to its high detection sensitivity in dilute solutions and the results that we have obtained to date suggest a broad range of potential applications for this exciting new technique.”
This new tandem SAW-ESI-MS system has been developed in the laboratory of Professor Michael Przybylski at the University of Konstanz and recently published (Dragusanu et al, 2010)1. Biosensors have previously only ever been connected to mass spec indirectly in a MALDI-TOF format where crystallised sample spots are analysed. The new system directly connects liquid flow from the SAW biosensor directly into the ESI-MS via a standard desalting interface – an approach impossible with other available biosensors. This novel set-up enables direct quantitative determinations of protein-ligand complexes by SAW-yielded dissociation constants (KD) from low nanomolar to sub-micromolar sample concentrations.
The Przybylski team observed the key advantages of SAW in comparison to classical immuno-analytical bioaffinity techniques to be the direct and rapid determination of association/dissociation constants from small and dilute sample amounts, without the need for labelling or recalibration for buffer changes. Furthermore, the sam5 biosensor is able to make detection measurements in complex biological samples e.g. crude brain samples, blood or cell lysates.
The new SAW-ESI-MS technique has been successfully applied by the Przybylski group to a number of clinically relevant applications for biomarker discovery, analysis and epitope mapping. These include human ß-amyloid peptides (Alzheimer’s Disease), Substance P peptide-calmodulin complex, tyrosine-nitrated peptides (neurotoxic events) and human ß-synuclein (Parkinson’s Disease). Indeed, no details on affinity binding and KD determination of the anti-a-synuclein-human a-synuclein complex have previously been reported.
“We are confident that the new on-line SAW-ESI-MS approach utilizing the sam5™ biosensor will develop into an efficient and sensitive tool for the label-free detection, identification and quantification of biopolymer-ligand interactions as diverse as antigen-antibody and lectin-carbohydrate complexes,” said Professor Michael Przybylski. “Certainly SAW technology is now becoming increasingly popular for the study of biomacromolecular interactions due to its high detection sensitivity in dilute solutions and the results that we have obtained to date suggest a broad range of potential applications for this exciting new technique.”
