Pipetted Liquid Sampling - Automated sample Preparation Platform for Online LC-MS/MS Bioanalysis

The SCAP PLS system samples directly from disposable cartridges without prior solid-phase extraction or any other sample desalting/pre-processing step. After placing the cartridges into the SCAP PLS system sample rack the samples may be stored in a freezer, shipped to another location or are directly analyzed. The cartridges are automatically picked-up by the autosampler gripper tool and are inserted into the clamp module. Both ends of the cartridge are pierced to eject the sample into the analytical flow path. The sample passes first through a pulsed ultrasonic module, where the blood cell components are disintegrated and homogenized. This patented technology avoids any clogging of the fluidic path or the SPE pre-columns. Pre-columns may be used up to 1000 extractions before exchange. Subsequently the analyte reaches a restricted access material column (RAM) to remove selectively higher molecular components such as proteins. The trapped lower molecular compounds are eluted again and finally separated on the analytical LC column.

Full details can be found in the SCAP PLS system brochure