Protein Discovery Inc. Announces new FASP™ Protein Digestion Kits
Product News Nov 24, 2010
Protein Discovery has announced the introduction of two new sample preparation kits designed to advance a "universal" sample preparation protocol for mass spectrometry-based proteome research.
Based on the Filter-Aided Sample Preparation method, Protein Discovery's FASP Protein Digestion Kits enable complete, reproducible solubilization and digestion of both fluid and tissue sample proteomes, and extend mass spectrometry-based proteome research methods into the previously inaccessible realms of the insoluble and FFPE (biobank) proteomes.
Comparative proteome studies, such as those required to develop clinical diagnostics, have been impeded by mass spectrometry data variability associated with incomplete proteome solubilization and incomplete proteolytic digestion problems affecting conventional sample preparation workflows.
Protein Discovery's FASP Protein Digestion Kits eliminate this source of variation by allowing researchers to completely solubilize the proteomes of fluid and tissue samples with the use of aggressive detergents, and to drive digestion reactions toward complete hydrolysis.
By enabling the use of strong detergents within mass spectrometry sample preparation workflows, Protein Discovery's new FASP Protein Digestion Kit offers a standardized sample preparation workflow that works for both soluble and insoluble proteins in fluids and tissues. Principal advantages of the FASP Protein Digestion Kit include complete proteome solubilization, sample cleanup with detergent depletion, and highly efficient proteolytic enzyme digestion of proteins.
The composition of Protein Discovery's new FFPE-FASP Protein Digestion Kit is similar to that of the FASP Protein Digestion Kit, but the FFPE-FASP Kit contains supplemental materials and protocol steps to guide researchers through additional tissue sample and protein treatment steps. These steps are required to ensure compatibility of FFPE (formalin-fixed paraffin-embedded) samples with downstream mass spectrometry sample preparation and analysis workflows.
As the principal sample format of biobank libraries, FFPE tissue samples represent a rich opportunity for productive proteome research, as many of these samples are associated with specific clinical histories and outcome measures. With the FFPE-FASP Protein Digestion Kit, researchers will be able to study how the human proteome responds and interacts with clinical events such as the initiation and discontinuation of drug therapy, and disease stage progression.
"We agree with researchers who believe that the only way to achieve an unbiased extraction and solubilize the entire proteome is to use a strong detergent like SDS," said Jeremy Norris, PhD, vice president of research and development at Protein Discovery.
"Scientists can now apply the productive mass spectrometry-based protein analysis methods developed over the past fifteen years to the great expanse of the proteome that isn't readily soluble in the weaker detergents they've had to use. And now that the technique can also be used with biobank library tissues, we'll be seeing more and more mass spectrometry studies with direct clinical relevance," said Dr. Norris.