We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.

Advertisement
Protein Glycosylation Evaluation in Less Than 10 µg of Protein Samples
Product News

Protein Glycosylation Evaluation in Less Than 10 µg of Protein Samples

Protein Glycosylation Evaluation in Less Than 10 µg of Protein Samples
Product News

Protein Glycosylation Evaluation in Less Than 10 µg of Protein Samples


Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "Protein Glycosylation Evaluation in Less Than 10 µg of Protein Samples"

First Name*
Last Name*
Email Address*
Country*
Company Type*
Job Function*
Would you like to receive further email communication from Technology Networks?

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Thermo Fisher Scientific has developed a new approach that combines high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) analysis, acid hydrolysis, and enzymatic digestion of protein glycans to determine information about glycan identity and terminal carbohydrate linkage isomers from small amounts of protein-just 0.5–1.6 μg per injection.

Application Note (AN) 1050: Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD demonstrates that a suite of HPAE-PAD analyses can be performed with sample quantities as low as 3-10 μg. This direct determination approach saves time and reagent costs.

Cancer research frequently involves studying changes in protein glycosylation to identify important biomarkers such as prostate-specific antigen (PSA).

The AN 1050 methodology uses an ion chromatography system configured for HPAE-PAD to investigate human PSA N-linked glycosylation and to evaluate the potential presence of O-linked glycans with methods optimized to work with small quantities of glycoproteins.

Advertisement