SIM Technology From MicroCal Revolutionizes ITC Throughput
Product News Nov 18, 2005
MicroCal, LLC has announced the development of the Single Injection Method (SIM) for its Isothermal Titration Calorimetry (ITC) systems: the VP-ITC and AutoITC.
This technique provides application flexibility and enables higher-throughput thermodynamic analysis of binding interactions than previously possible.
Modifications to the injection system and stirring base of the VP-ITC allow for a broader range of cell stirring speeds and injection rates that make SIM possible.
SIM technology provides a continuous, slow injection of ligand, producing differential power data which is converted into a binding isotherm. This is then fit to a binding model using a specific calorimetric analysis algorithm.
SIM has been demonstrated to measure binding affinities between 103 M-1 to 109 M-1 and the data generated is comparable to that from a multiple injection ITC run producing measurements of changes in enthalpy (†H) and entropy (†S), binding affinity (KB) and the stoichiometry of binding (n).
Depending on experimental conditions, a typical SIM experiment takes 20-50 minutes from the start of injection, whereas the same experiment using multi-injection methods requires 10-30 injections taking 45-120 minutes.
MicroCal™s ITC solutions provide an accurate thermodynamic profile characterizing the binding of any two biomolecules, such as: enzyme-inhibitor; protein-drug; protein-protein; antibody-antigen and protein-DNA.
This information rich data is obtained through the direct measurement of †H and the determination of †S, KB and n.