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SIM Technology From MicroCal Revolutionizes ITC Throughput

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MicroCal, LLC has announced the development of the Single Injection Method (SIM) for its Isothermal Titration Calorimetry (ITC) systems: the VP-ITC and AutoITC.

This technique provides application flexibility and enables higher-throughput thermodynamic analysis of binding interactions than previously possible.

Modifications to the injection system and stirring base of the VP-ITC allow for a broader range of cell stirring speeds and injection rates that make SIM possible.

SIM technology provides a continuous, slow injection of ligand, producing differential power data which is converted into a binding isotherm. This is then fit to a binding model using a specific calorimetric analysis algorithm.

SIM has been demonstrated to measure binding affinities between 103 M-1 to 109 M-1 and the data generated is comparable to that from a multiple injection ITC run producing measurements of changes in enthalpy (†H) and entropy (†S), binding affinity (KB) and the stoichiometry of binding (n).

Depending on experimental conditions, a typical SIM experiment takes 20-50 minutes from the start of injection, whereas the same experiment using multi-injection methods requires 10-30 injections taking 45-120 minutes.

MicroCal™s ITC solutions provide an accurate thermodynamic profile characterizing the binding of any two biomolecules, such as: enzyme-inhibitor; protein-drug; protein-protein; antibody-antigen and protein-DNA.

This information rich data is obtained through the direct measurement of †H and the determination of †S, KB and n.