Technical Note for Determination of Water- and Fat-Soluble Vitamins
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The document also describes a method that allows for the determination of fat- and water-soluble vitamins in one analysis with a run time of 25 minutes using the Dionex UltiMate® 3000 dual system and Acclaim® reversed-phase columns.
The dietary supplement industry has been unregulated in the US until recently. In June 2010, the dietary supplement safety act went into affect requiring all dietary supplement manufacturers and importers to adhere to current Good Manufacturing Practices (cGMP). The industry now needs to ensure that the product is safe for consumers and meets label claims, which entails raw material and final product testing.
Due to the wide range of physical-chemical properties of vitamins, sample types, and concentrations, there is no universal vitamin determination method. Technical Note 89 provides information that will help customers in the food, nutritional, and pharmaceutical industries develop their own vitamin determination method.
