We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.


Thermo Fisher Scientific Develops Method to Eliminate High Boiling Matrix Contamination in GC and GC/MS Analyses of Pesticides in Food

Want a FREE PDF version of This Product News?

Complete the form below and we will email you a PDF version of "Thermo Fisher Scientific Develops Method to Eliminate High Boiling Matrix Contamination in GC and GC/MS Analyses of Pesticides in Food"

Technology Networks Ltd. needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy

Thermo Fisher Scientific Inc., has announced a new method to effectively eliminate invisible high boiling matrices in the analysis of pesticides in food. Incorporating a programmable temperature vaporizing (PTV) injector, the Thermo Scientific TRACE GC Ultra GC/MS analyzer achieves sensitive, fast and reliable analysis of pesticides in low fat food products, significantly enhancing the productivity and efficiency of gas chromatography (GC) and gas chromatography mass spectrometry (GC/MS) systems for analyzing pesticides in food, while eradicating high boiling matrix.

Pesticide extraction in low-fat food products, such as fruits and vegetables, normally results in high concentrations of lipid components as a matrix of high boiling compounds in the extracts. Once injected into a GC or GC/MS analyzer, high boiling substances accumulate on the analytical column of the system, contaminating it and causing an increasingly high background level.

As high boiling compounds cannot be seen, there is no possibility of visual quality control. Bake-out procedures have been traditionally used, but these methods increase time between samples, are inefficient and reduce the column lifetime. An optimum solution would be the separation of the analytes from all high-boiling matrix material directly after injection.

The new application from Thermo Fisher demonstrates that a PTV injector with a pre-column and a carrier gas backflush capability offers a powerful method for separating analytes from high-boiling compounds. Following sample extraction using the QuEChERS technique, a PTV injector was used to inject the extract into the Thermo Scientific TRACE GC Ultra GC/MS analyzer.

Pesticides travelled quickly into the system's analytical column whereas high boilers were kept in the pre-column that was swept backwards concurrently during the analytical run. As a result, the analytes transferred to the column and eluting to the MS source were free from high molecular weight compounds.

Experimental results demonstrated that the PTV-GC/MS system can be used for both regular and large volume injections with numerous productivity advantages, including increased sensitivity, time and cost savings, easy maintenance, increased column lifetime and higher robustness of the entire analytical system.