Insider Tips for GPCR signaling studies & phospho-Erk

G protein-coupled receptors (GPCRs) are among the most abundant proteins in the body, and responsible for triggering numerous and diverse signaling cascades. Signaling through the MAPK/ERK pathway is activated through a variety of external stimuli, dependent on the G protein subtype coupled to the GPCR. Detecting ERK1/2 phosphorylation mediated by endogenous or overexpressed GPCRs is valuable for cellular research and drug discovery efforts, but offers challenges due to the abundance of related signaling pathways. The HTRF® Advanced phospho-ERK1/2 kit is a highly sensitive and reliable tool for detecting ERK1/2 phosphorylation mediated by GRPCs in various cellular models. This technical note offers key tips and easy optimization techniques to help you ensure optimal results from your GPCR signaling studies. Download this complimentary technical note today.