ELISA – Multimedia

In this video, the Analytical Development group at AGC Biologics Milan describes the automation of two ELISA-based workflows to assess lentiviral vector purity, using a Hamilton Microlab® STARlet liquid handler integrated with a plate washer, a plate spectrometer, and two plate incubators.

Bioassays, such as the enzyme-linked bioabsorbant assay (ELISA), are standard techniques that require enzyme or fluorescent labels for the detection of target analytes. While these label-based studies give important information about a sample, such as binding affinity, they can only measure the interaction of an analyte at a given concentration and time, and are unable to gather kinetic analysis.

Download this eBook to learn about technology-driven solutions that perform faster analysis – with fewer sample preparation steps.

In quantifying therapeutic peptides and proteins in biological matrices, traditional ELISA-only based techniques can suffer from high variability, narrow dynamic range, problems with selectivity and typically cannot use internal standards.

Download this guide to learn more about the advantages of multiplex immunoassays, the importance of multiplexing for drug discovery as well as how to choose your multiplex assay for use in drug discovery.

Enzyme Linked Immunosorbent Assays (ELISAs) are commonly used to quantify biomarkers in serum, plasma and cell culture supernates. These samples contain a variety of factors that can interfere with ELISA results, commonly referred to as a matrix effect. Eliminating these factors is essential to obtaining accurate sample values.

Download your guide to learn the answers to commonly asked questions such as what is at the core of your immunoassay as well as why use an ELISA over other techniques?

Download this poster to discover tips on moisture prevention, incubation times and dispensing techniques.

Download this app note to explore various solutions designed to improve COVID-19 detection and the development of vaccines and therapeutics.

At high levels, host cell proteins (HCPs) can cause immunogenic responses and reduced efficacy of biologics, so it is imperative to have an effective, robust HCP assay to detect them and mitigate these effects.