Western Blot – Multimedia

Follow these 7 top tips to improve your westerns and reap the rewards of clean bands and publishable quality data, every time.

The following is a list of what I think are the most useful tips for successful transfection, learned through years of experience and troubleshooting transfection experiments.

Abnormal tau hyperphosphorylation and accumulation into neuronal neurofibrillary tangles are linked to neurodegeneration in Alzheimer’s disease. The aim of this study was to test the effects of a new therapeutic agent in a mouse model of tau deposition (Tg4510 mice). Mice treated with test agent-1 showed improvements in learning and memory compared to the control mice together with a reduction in tau oligomers in hippocampi homogenates.

PrecisionAb™ Validated Western Blotting Antibodies are our solution to the current pain points of many protein scientists looking for high-quality, trustworthy antibodies to detect their protein of interest with minimal optimization.

For a primary antibody to be selected as a PrecisionAb Antibody, it must be highly specific, highly sensitive, and pass a rigorous validation process that includes consistent performance from batch to batch.

Watch this video to see how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

These are premium-quality antibodies extensively validated for western blotting.

The generally low quality of western blotting data has led scientists to question whether we can trust protein quantification when using western blots.

This video demonstrates SDS-PAGE separation of proteins using the Bio-Rad Comparative Proteomics Kit II: Western Blot Module.

Because of the possible beneficial role of polyamines, metabolism of arginine by arginase is of interest. Recently, our lab has recently shown that arginase1 overexpression has the ability to reduce phosphorylated tau and neurofibrillary tangles in rTg4510 and PS19 tau transgenic mice. Based on these data, we utilized the transgenic tetO MAPT*P301L mouse model to examine a potential therapeutic gene of interest, arginase1, in an animal model of tauopathy.