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Microinjecting CRISPR: Options and key considerations for creating transgenic animal models content piece image
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Microinjecting CRISPR: Options and key considerations for creating transgenic animal models

CRISPR is revolutionizing the life sciences. Genome editing using RNA-programmable nucleases opens up a wealth of experimental options. At the same time, it makes creating new cell and animal models faster, easier and cheaper than in the past. Consequently, researchers in areas as diverse as cell/developmental biology, genetics or clinical research are turning to CRISPR as their method of choice.
Detecting volatile food contaminants content piece image
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Detecting volatile food contaminants

We spoke to Andrea Buettner, Professor, Fraunhofer IVV, at Food Safety & Analysis 2016.
Detection and Characterization of Nanoparticles in Food and Biological Materials content piece image
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Detection and Characterization of Nanoparticles in Food and Biological Materials

With the increasing use of nanotechnology in food and consumer products, there is a need for reliable detection and characterization methods for nanoparticles (NPs) in complex matrices. NPs often interact with each other or with their surroundings leading to aggregation, adhesion to surfaces or dissolution in dispersion solvents. Accurate and precise characterization of metrics such as size, shape, particle mass and number concentration therefore remains a challenging analytical task. Asymmetric flow field flow fractionation (AF4) hyphenated with inductively coupled plasma mass spectrometry (ICP-MS) is a powerful technique for the detection and characterization of inorganic NPs in complex matrices. In order to acquire accurate data the AF4 separation method and settings must be optimized for each new sample matrix and analyte NP combination. Furthermore, additional information obtained by single particle (sp) ICP-MS analysis of collected fractions or an imaging method such as transmission electron microscopy (TEM) are necessary for trouble shooting and for independent verification of results. spICP-MS as stand-alone technique is a powerful screening method for many inorganic NPs. A number of examples where AF4-ICP-MS and single particle ICP-MS were applied for the detection and characterization of NPs in food and biological matrices will be presented including: • Silver NPs in chicken meat • Silicon dioxide NPs in tomato soup • Aluminum-containing NPs in noodles • Gold NPs in cells
Quantitative Top-Down Proteomics & Its Impact on Clinical Research/Basic Biology content piece image
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Quantitative Top-Down Proteomics & Its Impact on Clinical Research/Basic Biology

Listen as Prof Neil Kelleher, discusses how he believes the Orbitrap Fusion Lumos MS might better characterize unexpected PTMs in Top-Down Proteomics to decode cellular function.
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Horizon Discovery: The Power of Personalized Medicine

Learn about how Personalized Medicine is changing how drugs are being developed and how healthcare is being delivered, and how Horizon is helping to enable this change.
Behind the Science, ep. 13: SPE vs. matrix effects – battle episode! content piece image
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Behind the Science, ep. 13: SPE vs. matrix effects – battle episode!

Cardiac iPSCs for Drug Discovery content piece image
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Cardiac iPSCs for Drug Discovery

Characterizing the Performance of qPCR Instruments  content piece image
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Characterizing the Performance of qPCR Instruments

DeNovix DS-11 FX Spectrophotometer / Fluorometer Series:  1uL UV-Vis + Fluorescence content piece image
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DeNovix DS-11 FX Spectrophotometer / Fluorometer Series: 1uL UV-Vis + Fluorescence

LabTube Meets John Draper, Aberystwyth University content piece image
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LabTube Meets John Draper, Aberystwyth University

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