We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience, read our Cookie Policy

NMR Spectroscopy

Video   Dec 17, 2018


NMR spectroscopy lecture by Suman Bhattacharjee - This lecture explains about the NMR spectroscopy basics. Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy, is a research technique that exploits the magnetic properties of certain atomic nuclei. It determines the physical and chemical properties of atoms or the molecules in which they are contained. It relies on the phenomenon of nuclear magnetic resonance and can provide detailed information about the structure, dynamics, reaction state, and chemical environment of molecules. The intramolecular magnetic field around an atom in a molecule changes the resonance frequency, thus giving access to details of the electronic structure of a molecule.

Most frequently, NMR spectroscopy is used by chemists and biochemists to investigate the properties of organic molecules, although it is applicable to any kind of sample that contains nuclei possessing spin. Suitable samples range from small compounds analyzed with 1-dimensional proton or carbon-13 NMR spectroscopy to large proteins or nucleic acids using 3 or 4-dimensional techniques. The impact of NMR spectroscopy on the sciences has been substantial because of the range of information and the diversity of samples, including solutions and solids.

NMR spectra are unique, well-resolved, analytically tractable and often highly predictable for small molecules. Thus, in organic chemistry practice, NMR analysis is used to confirm the identity of a substance. Different functional groups are obviously distinguishable, and identical functional groups with differing neighboring substituents still give distinguishable signals. NMR has largely replaced traditional wet chemistry tests such as color reagents for identification. A disadvantage is that a relatively large amount, 2–50 mg, of a purified substance is required, although it may be recovered. Preferably, the sample should be dissolved in a solvent, because NMR analysis of solids requires a dedicated MAS machine and may not give equally well-resolved spectra. The timescale of NMR is relatively long, and thus it is not suitable for observing fast phenomena, producing only an averaged spectrum. Although large amounts of impurities do show on an NMR spectrum, better methods exist for detecting impurities, as NMR is inherently not very sensitive.

NMR spectrometers are relatively expensive; universities usually have them, but they are less common in private companies. Modern NMR spectrometers have a very strong, large and expensive liquid helium-cooled superconducting magnet, because resolution directly depends on magnetic field strength. Less expensive machines using permanent magnets and lower resolution are also available, which still give sufficient performance for certain application such as reaction monitoring and quick checking of samples. There are even benchtop NMR spectrometers.

This important and well-established application of nuclear magnetic resonance will serve to illustrate some of the novel aspects of this method. To begin with, the nmr spectrometer must be tuned to a specific nucleus, in this case the proton. The actual procedure for obtaining the spectrum varies, but the simplest is referred to as the continuous wave (CW) method. A typical CW-spectrometer is shown in the following diagram. 

More Information

Recommended Videos

How Do Scientists Know if Plastic Containers are BPA-free? | Behind the Science, S3 Ep5


How do consumer packaging companies test their products to maintain "BPA-free" label claims? VICAM, a division of Waters that develops rapid diagnostics and laboratory sample prep technologies for testing quality and safety in food and agriculture, has a new fit-for-purpose BPA test that pairs immunoaffinity chromatography to extract and elute BPA for subsequent measurement by LC-MS/MS.


Your Fingerprints Reveal More than You Think


Our fingerprints are what make us unique -- but they're also home to a world of information hidden in molecules that reveal our actions, lifestyles and routines.


A Crash Course in Organic Chemistry


Jakob Magolan is here to change your perception of organic chemistry. In an accessible talk packed with striking graphics, he teaches us the basics while breaking the stereotype that organic chemistry is something to be afraid of.



Like what you just watched? You can find similar content on the communities below.

Applied Sciences

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free