Peptide Mass Fingerprinting

Video   Oct 17, 2017

 

Peptide Mass Fingerprinting (PMF) is an analytical technique for identifying unknown protein. Proteins to be identified is first digested into peptides, whose absolute mass can be accurately analyzed by a mass spectrometer, such as MALDI-TOF or ESI-TOF. MALDI is more preferred in PMF analysis, as it has a wide sequence coverage and can analyze a large amount of sample at the same time. The peptide mass are compared to database containing the theoretical peptide masses of all known protein sequences.

 
More Information
 
 
 

Recommended Videos

Why Protein Structure Matters in Drug Development

Video

Dr. Steven Almo explains in lay terms why protein structure and shape are important in developing drugs to fight disease.

WATCH NOW

Behind the Science, S2 Ep9: Gareth Cleland on how to tell if basmati rice is authentic

Video

Gareth discusses his analytical workflow for detecting adulterated foods, and how mass spectrometry and informatics are used together in both research and routine authenticity studies.

WATCH NOW

NanoDrop One UV-Vis Spectrophotometer Review – Alyssa Grantham

Video

See what an experienced, young scientist working on amyotrophic lateral sclerosis (ALS) research has to say about the Thermo Scientific™ NanoDrop™ One.

WATCH NOW

 

Comments | 0 ADD COMMENT

Like what you just watched? You can find similar content on the communities below.

Proteomics & Metabolomics

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE