Trust Your Science: Are all C18 (L1) columns the same?

Columns specified in USP monographs are usually designated by an “L” classification; for example, L-1 refers to columns bonded with a C18 functional group. Many scientists expect equivalent retention and selectivity from columns with the same L designation. In this episode of Trust Your Science, we evaluate a number of C18 columns under identical conditions, to either prove or disprove if these columns are truly equivalent. Kim Haynes and Jonathan Turner are chromatography experts at Waters.

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