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Trust Your Science: How does carbon load on a column relate to retention?

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Video

Trust Your Science: How does carbon load on a column relate to retention?

Have you ever heard that higher carbon loads on a chromatographic column lead to more retention? Or, have you heard that column equivalency is driven by matching percent carbon between columns?

Columns specified in compendial monographs are typically designated by the bonded phase and percent carbon value. Many scientists expect equivalent retention between columns with equivalent % carbon values. In this episode of Trust Your Science, we take a look at the impact the percent carbon value has on chromatographic retention, to prove or disprove if there truly is a correlation between the two. Kim Haynes and Jonathan Turner are chromatography experts at Waters.

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