What is Mixed-Mode Chromatography?

Video   Apr 11, 2017

 

This video reviews the advantages of using mixed-mode chromatography media. Mixed-mode chromatography depends on more than one interaction principle occurring between stationary phase and solute.

Mixed-mode chromatography media are generally designed with the addition of a spacer and ligand to a bead matrix. The ligand design impacts the strength and specificity of the interactions. Multiple interactions between stationary and mobile phases can lead to unique selectivity and facilitate separation of closely related proteins and contaminants.

Advantages of mixed-mode separations include
· Greater resolution compared to unimodal interactions · Improved yield and activity via advantageous use of charge-charge repulsion between ligand and protein
· Large design space for protein binding
· Minimal feed manipulation prior to binding
· Mild operating conditions preserve activity Bio-Rad Process Separations offers a full range of chromatography solutions for biomolecule purifications.

 
More Information
 
 
 

Recommended Videos

What Are Pesticides and How Do They Get Into Our Food?

Video

Pesticides play an important role in maintaining yields of agricultural crops. However, the residues from pesticides can potentially be harmful to humans if they get into the food we eat. A pesticides expert at EFSA explains the measures that are in place to reduce the risks.

WATCH NOW

How Do Our Bodies Fight Off Dangerous Chemicals?

Video

UC San Diego's Amro Hamdoun explains how you can think of cells like night clubs. With every substance that a cell encounters it has to decide which to eliminate and which to let in. Since humans have generated over 80,000 synthetic compounds, it's now ever more important to.

WATCH NOW

Lumpy Skin Disease Vaccination Programme in Europe

Video

In January 2016 a number of countries in South East Europe started vaccinating cattle against lumpy skin disease. As the programme has progressed the number of outbreaks has fallen and by the end of 2017 the outbreaks had been contained.

WATCH NOW

 

Comments | 0 ADD COMMENT

To personalize the content you see on Technology Networks homepage, Log In or Subscribe for Free

LOGIN SUBSCRIBE FOR FREE