What is Mixed-Mode Chromatography?
Video Apr 11, 2017
This video reviews the advantages of using mixed-mode chromatography media. Mixed-mode chromatography depends on more than one interaction principle occurring between stationary phase and solute.
Mixed-mode chromatography media are generally designed with the addition of a spacer and ligand to a bead matrix. The ligand design impacts the strength and specificity of the interactions. Multiple interactions between stationary and mobile phases can lead to unique selectivity and facilitate separation of closely related proteins and contaminants.
Advantages of mixed-mode separations include
· Greater resolution compared to unimodal interactions · Improved yield and activity via advantageous use of charge-charge repulsion between ligand and protein
· Large design space for protein binding
· Minimal feed manipulation prior to binding
· Mild operating conditions preserve activity Bio-Rad Process Separations offers a full range of chromatography solutions for biomolecule purifications.
Behind the Science, S2-Ep1: Classic rock & HPLC with Pat McDonaldVideo
Every good rock band starts in the basement. So that's where we're heading ... to the basement of Waters to see the first chromatography systems! Pat McDonald, corporate fellow emeritus at Waters, tells us about Jim Waters and Waters Associates' inventions.
The first commercial "high pressure" chromatography system for polymer chemists, the GPC-100, from 1963. The ALC-100, developed in late 1970 for separating isomers in the synthesis of B12. The M6000 pump that came in 1972, followed by the U6K injector, the first commercial "small" particle columns (10 µm!). Bringing those pieces into an assembly led to the first modern "high performance" liquid chromatography instrument, now known as HPLC.
Read up on the history of Jim Waters and his chromatography people: http://bit.ly/2mwT2BI
Hair Strands Could Reveal Lifestyle Secrets of SuspectsVideo
Hair fiber analysis could soon have a brighter future thanks to the development of a more refined scientific technique.WATCH NOW