Absolute Biophysical Characterization with Multi-Angle and Dynamic Light Scattering
Traditional analytical size exclusion chromatography (SEC) is limited to determining molecular weight distributions of molecules that are similar to the reference proteins or polymers used to calibrate the column. Molecules with different conformations or surface interactions (charged, hydrophobic, etc.) will elute differently, leading to incorrect results.
Multi-angle light scattering (MALS) and dynamic light scattering (DLS) resolve those inherent deficiencies by providing first-principles characterization of molecular weight, size and conformation, independent of column calibration; the SEC column is only utilized to separate the solution components in order to obtain accurate distributions. A SEC-MALS-DLS system extends the characterization range of SEC beyond ideal, well-behaved globular proteins or linear polymers to include glycoproteins, PEGylated proteins, hydrophobic proteins, and branched polymers, for full confidence in biophysical characterization results.