Achieving Accurate Results with LC-MS/MS for Aldosterone, Plasma Renin Activity, and Cortisol
Dr. Daniel T. Holmes
Division Head, Clinical Chemistry and Assistant Head, Department of Pathology and Laboratory Medicine, St. Paul’s Hospital
Primary Aldosteronism (PA) is the most common form of secondary hypertension accounting for up to 10% of all-cause hypertension in unselected populations. In the majority of cases, PA is caused by adrenal hyperplasia or aldosterone producing adenoma of the adrenal gland. If left untreated, PA commonly results in the well-documented consequences of unchecked elevations in blood pressure.
Measurement of aldosterone and plasma renin activity can both be achieved by HPLC and tandem mass spectrometry (LC-MS/MS) using a sufficiently sensitive instrument. However, by using LC-MS/MS technology, we discovered an improvement in analytical performance over traditional methods and identification of alterations in the concentrations of relevant compounds.
This webinar will discuss how aldosterone and plasma renin activity can be performed using a streamlined sample-prep followed by LC-MS/MS, the capability of co-measuring plasma cortisol, and the numerous benefits of LC-MS/MS.
- How to recognize the aspects of LC-MS/MS method development and validation necessary to achieve accurate results for aldosterone, plasma renin, and cortisol measurement.
- How to Identify the improved analytical performance of LC-MS/MS technology for the measurement of aldosterone, plasma renin activity, and cortisol.
- How to Recall the sensitivity requirements for LC-MS/MS to accurately measure aldosterone, plasma renin activity, and cortisol.