Applications of Targeted Mass Spectrometry Assays for Quantitative Analysis of Cancer Signaling Proteins
Bhavin Patel, MD, MS
R&D Scientist, Protein and Cell Analysis, Thermo Fisher Scientific
Jonathan Krieger, PhD.
Lab Research Project Manager/Mass Spectrometry Applications Specialist, SPARC Biocentre, The Hospital for Sick Children, Toronto, Canada
Proteomics offers a deeper understanding of the protein events influencing various cellular and biological functions. However, identifying key regulated proteins and their implicated signaling pathways represents a major analytical challenge to researchers. This is due to the broad dynamic range of protein abundance in biological samples and the inherent limitations of the instrumentation and methodologies utilized to probe and detect minute target proteins in complex cellular environments.
In this webinar, we will discuss targeted mass spectrometry (MS) based approaches and the various strategies that are designed to reduce sample complexity through improved sample preparation and enrichment. Focusing on proteins involved in the AKT/mTOR signaling pathway, we will provide guidance towards targeted MS assay development and verification, including robustness, precision, and accuracy of these assays. Finally, we will examine how the results of multiplexed immunoprecipitation to targeted MS assays compare to existing immunoassay techniques.
Attend this webinar to:
Learn about the development and verification of immunoprecipitation to mass spectrometry (IP-MS) assays
Gain insights into the accurate and precise quantitation of multiple AKT/mTOR pathway proteins by multiplexed IP to targeted MS assays
Learn how multiplexed IP to targeted MS assays compare to existing immunoassay techniques.