Identification of Protein Aggregation Through UV-Visible Spectroscopy
Protein aggregates can irreversibly form in solution through a number of processes and are common within unstable samples. This results in a lower concentration of non-aggregated materials which can negatively impact pharmaceutical applications and can require further purification steps. Therefore, it is important to determine the extent to which a sample is aggregated early in the experiment. Typically, aggregates are identified using size exclusion chromatography. However, recovering the samples after this analysis is difficult and often time-consuming.
Watch this webinar to learn about how:
- To rapidly recognize the presence of protein aggregates using UV-VIS
- The absorption of a protein aggregate can be obtained using an integrating sphere
- To determine the concentration of aggregated and non-aggregated materials in a scattering solution