Improved understanding of the thermal stability of a monoclonal antibody and its modified version by use of DLS/Raman spectroscopy
White Paper Jul 10, 2014
The combination of Dynamic Light Scattering (DLS) with Raman Spectroscopy provides the capability to investigate a wealth of chemical, structural, and physical parameters about biotherapeutic proteins under formulation conditions.
Here, we demonstrate that by simultaneously monitoring both the colloidal (DLS) and conformational (Raman) stability of a template monoclonal antibody (mAb) and a modified monoclonal antibody (mmAb) provides significant insights into the mechanistic effects of thermal stress on the mAb and the mmAb.
In particular, this combined DLS/Raman technique has revealed that the lower thermal stability (early oligomerization) of the mmAb compared to the template mAb, is initiated by a tertiary structural change in a tyrosine side chain residue; a mechanistic observation that could not have been revealed fully by the separate use of these techniques.
Related White Papers
8th Edition of International Conference and Exhibition on Separation Techniques
Jul 29 - Jul 30, 2019
International Women Health and Breast Cancer Conference
Jul 03 - Jul 05, 2019