The Effects of Mismatches on DNA Capture by Hybridization

Targeted next-generation sequencing (NGS) focuses the sequencing effort on specific regions by capturing and enriching them with complementary biotinylated probes. Though many factors can influence the efficiency of such capture, a primary consideration is how well the DNA sequence of the probe matches the target (sequence complementarity), as this affects both the efficiency and selectivity of capture.

Download this whitepaper to learn more about:

• The effects of sequence complementarity and mismatches on capture efficiency 
• The factors that might influence probe design