Analysis of Fat-Soluble Vitamins from Food Matrix for Nutrition Labeling

This Application Note shows how to carry out qualitative and quantitative analysis of fat soluble vitamins from two different food matrixes. A robust reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous determination of nine fat soluble vitamins was developed. Separation and quantifi-cation was achieved by an Agilent 1260 Infinity LC System using an Agilent Poroshell EC-C18 column. The advanced feature of the Agilent 1260 Infinity Diode Array Detector (DAD) to select multiple wavelengths was utilized effectively to detect various vitamins at their maximum absorbance. Robustness of the method for routine nutrition labelling analysis was established by partial validation and the method was verified by analyzing vitamin D content from two different food matrices. Finally, using an Agilent 1290 Infinity LC System, this HPLC method was effectively transferred to a short Ultra High Pressure Liquid Chromatographic (UHPLC) method.