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Determination of KD of Aptamer Protein Interactions by Microfluidic Diffusional Sizing

Determination of KD of Aptamer Protein Interactions by Microfluidic Diffusional Sizing content piece image

The interactions of proteins with secondary molecules are of key importance across the life sciences.

While methods exist to determine KD experimentally, these often require a degree of expertise to collect reliable data and can have technique specific limitations.

This application note presents a new, simple and rapid technique to characterize protein interactions in vitro; microfluidic diffusional sizing (MDS).

Download this application note to learn a simple means to experimentally determine KD:

  • Without the need to alter molecules
  • No surface fixing
  • No complex preparation steps