Determine Kinetics Earlier in the Drug Discovery Process
Determination of accurate binding kinetics and affinity along with other critical quality attributes (CQAs) plays an ever-increasingly important role in identifying and isolating therapeutic molecules.
During high-throughput screening (HTS), drug candidates are typically prepared as a single concentration and injected across the target. A major restriction of this approach is that no quantitative information about kinetic parameters can be determined and that any hits need subsequent characterization using a more laborious multi-concentration assay.
This app note highlights how reliable kinetics for high-affinity interactions can be obtained from single injections.
Download this app note to learn more about:
- Generating accurate binding kinetics from a single concentration gradient
- Reducing sample preparation with lower analyte concentrations
- A cost-effective method for the early drug discovery process