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Fast Determination of Anthocyanins in Pomegranate Juice
Application Note

Fast Determination of Anthocyanins in Pomegranate Juice

Fast Determination of Anthocyanins in Pomegranate Juice
Application Note

Fast Determination of Anthocyanins in Pomegranate Juice

The method described in this application note is a sensitive, fast, and accurate way to determine anthocyanins in commercially available fruit juices using a simple dilution. Anthocyanins were separated using a 2.2 μm, Acclaim® RSLC 120, C18 rapid separation liquid chromatography column and detected at a visible wavelength of 540 nm. The silica-based column used in this application is designed for rapid, high-resolution separations, which is compatible with ultrahigh pressure instrumentation. The six anthocyanins of interest were separated in <5 min in various beverages that included PJ, grape juice, simulated adulterated PJ, pomegranate cherry juice, and pomegranate wildberry juice.

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