HPLC Autosampler Performance I: Challenging USP Methods on the Alliance™ iS HPLC System

Challenging method conditions can impact autosampler performance on High-Performance Liquid Chromatography (HPLC) systems. Methods with highly organic sample diluents, coupled with low injection volumes, can adversely impact injection precision of an autosampler, which may lead to failure to meet system suitability requirements for routine assays. In this study, the injection precision of an Alliance iS HPLC System was evaluated using four compendial HPLC methods from the United States Pharmacopeia (USP) with challenging method conditions and strict system suitability criteria. Peak area reproducibility of the selected compounds was evaluated as a proxy for autosampler performance.

Benefits
- Demonstrates high precision for a range of sample diluents and injection volumes
- Meets strict system suitability criteria for challenging USP methods