HPLC Autosampler Performance II: Improved Injection Precision of USP Methods With the Alliance™ iS HPLC System

Method conditions and instrument characteristics can affect the autosampler performance of High-Performance Liquid Chromatography (HPLC) systems. Challenging method conditions, such as low injection volume and highly organic sample diluents, require high precision to meet strict suitability requirements. Instrument characteristics, such as sample aspiration mechanism and injector draw rate, often vary between vendors and may impact the precision performance of the autosampler. This can pose a challenge for analytical laboratories that desire to run methods with strict precision suitability criteria across HPLC systems from different vendors. In this study, the injection precision performance of the Alliance iS HPLC System and similar HPLC systems were compared using four compendial HPLC methods from the United States Pharmacopeia (USP) with strict precision criteria and challenging method conditions. Peak area reproducibility was evaluated as a proxy for autosampler performance of each system.

Benefits
- High precision for a range of sample diluents and injection volumes on the Alliance iS HPLC System
- Meets strict injection precision criteria for challenging USP methods on the Alliance iS HPLC System
- Demonstrates improved precision on the Alliance iS HPLC System over similar HPLC systems for highly organic sample diluent