Metabolic Preconditioning for Improved CAR-T Cell Therapy

Metabolism is a key driver of CAR-T cell fitness and function. Hence, metabolic preconditioning methods can enhance the bioenergetic capacity of CAR-T cells in culture, supporting their persistence and antitumor activity.

This app note presents an optimized, in vitro approach for the characterization of CAR-T cell metabolic preconditioning. It demonstrates cell potency and bioenergetic assays that can be used to measure critical process parameters in the manufacturing of effective CAR-T cell therapies. 

Download this app note to learn about a workflow that:

• Provides confirmatory results from a single, simple approach
• Yields quantitative information about the killing kinetics and metabolic profile of preconditioned CAR-T cells 
• Delivers real-time potency results with minimal hands-on setup.