Modernization of a Legacy Normal-Phase HPLC Method

Legacy methods developed during the early days of HPLC technology have not kept pace with recent advancements and modernizations in the field. Advancements in column chemistry and system robustness now allow for better separation and detection of analytes. In this study, the United States Pharmacopeia (USP) propofol assay normal-phase method was successfully modernized on the Alliance™ iS HPLC PDA System using a modern column. All systems met suitability requirements and demonstrated comparable results.

Benefits
- Seamless normal-phase method modernization on the Alliance iS HPLC PDA System
- XBridge™ Column technology for improved performance of USP propofol method
- Scaling column dimensions within USP guidelines for lower solvent consumption and reduced waste