Rapid point of use Grouper Chek™ assay using the Douglas Scientific® AmpliFire® detects seafood fraud

The AmpliFire by Douglas Scientific along with GrouperChek isothermal amplification chemistry from PureMolecular, LLC provide a simple and portable tool to perform genetic analysis at the point of use. This paper describes a proof-of-concept experiment demonstrating the ability of the AmpliFire combined with the GrouperChek assay to differentiate the highly prized grouper from tilapia, a common illegal substitute.