Dynal Bead Introduces Immunoassay Bead for IVD Applications
Complete the form below to unlock access to ALL audio articles.
Dynal Bead Based Separations (part of the Invitrogen Group), has announced the introduction of a new immunoassay bead for in vitro diagnostic (IVD) applications – Dynabeads® MyOne™ Immunoassay (Carboxyl).
This bead type has been carefully developed to provide highly precise immobilization of small antigens such as peptides, steroids and hormones to the surface of the beads.
Hege Skjellerudsveen, Dynal IVD Product Manager said, “The new low charge carboxyl immunoassay bead, expands our excellent IVD bead offering.
It is particularly suited to infectious disease testing, along with other antibody tests (autoimmune, allergy, small molecule hormones and drugs).
It gives customers the opportunity to develop antigen-based assays more easily, as well as with much higher consistency and sensitivity compared to alternative techniques.”
The beads are functionalized with a controlled number of carboxylic acid groups.
The antigen of choice can then be coupled to the beads, either directly or via a linker, using carbodiimide mediated amide bond formation, which has been extensively discussed in the literature.
This also reduces the need for BSA blocking which is often used to avoid non-specific binding of serum proteins that would otherwise interfere with the assay.
Dynabeads MyOne™ Immunoassay (Carboxyl) provide an excellent solid support for antigen-down immunoassays. Their hydrophilic surface ensures low non-specific binding, excellent dispersion abilities and easy handling in a wide variety of buffers.
These beads also have a high magnetic mobility in combination with a low sedimentation rate, making them ideal for automated assays.
Furthermore, Dynabeads do not inhibit enzymatic activity and can be included directly in downstream analysis of the bead-bound target molecule. Alternatively, the target molecule can be eluted off the Dynabeads using conventional elution methods.
