Immunochromatographic Assay for Rapid Salmonella Detection
News Aug 12, 2014
An immunochromatographic assay was developed to detect Salmonella enterica serovars Typhimurium and Enteritidis in a single strip. The assay was constructed in the form of a sandwich, using 2 specific anti-S. Typhimurium and anti-S. Enteritidis antibodies immobilized on a nitrocellulose membrane at separated test lines, while the other specific antibody to Salmonella spp. was conjugated with gold nanoparticles. The test strips can immediately detect S. Typhimurium and S. Enteritidis specifically in a culture medium at levels as low as 10(4) and 10(6) cfu/ml, respectively. The contamination of S. Typhimurium and S. Enteritidis at 1 cfu/ml or greater can be detected by the test strips after 6-24 hr incubation. The specificities of the test strips to detect S. Typhimurium and S. Enteritidis, in spiked samples, were obtained at 100%; the sensitivities were at 98.89% (89/90) and 87.50% (70/80), respectively, compared with the conventional method. The newly developed multipleximmunochromatographic assay is the first report on the efficient detection of both S. Typhimurium and S. Enteritidis simultaneously in single strip. This test strip also provides advantages of simplicity and very rapid detection of these specific bacterial contaminants in chicken and can be useful for mass detection on chicken farms and in other veterinary products.
The article, Evaluation of an immunochromatographic assay for rapid detection of Salmonella enterica serovars Typhimurium and Enteritidis, is published online in the Journal of Veterinary Diagnostic Investigation and is free to access.
Computing Power Aids Fight Against Food PoisoningNews
In a proof-of-concept study, researchers have pinpointed new compounds that may be effective in containing the virulence – or ability to produce disease – of Listeria, a well-known bacterium that can cause severe food poisoning and even death.READ MORE
Babies Fed Soy-Based Formula Show Reproductive System ChangesNews
Infants who consumed soy-based formula as newborns had differences in some reproductive-system cells and tissues, compared to those who used cow-milk formula or were breastfed, according to a new study.READ MORE
Comments | 0 ADD COMMENT
10th International Conference on Mass Spectrometry and Chromatography
Oct 08 - Oct 09, 2018