Listeria Can Hide Inside Romaine Lettuce Tissue
Research led by Amanda Deering, clinical assistant professor in the Department of Food Science at Purdue University, showed that the bacteria could live within lettuce in every stage of the plant growth process, residing inside the plant tissue. L. monocytogenes can gain entry into the plant through cracked seed coats, small tears in root tissue during germination and damaged plant tissue. The researchers found that exposing lettuce to the bacteria could lead to infection of plant tissue in as little as 30 minutes.
“Knowing this can happen, we need to keep it on our radar as we continue to follow good agricultural practices,” Deering said.
When ingested, the bacteria can be deadly to those with vulnerable immune systems, including pregnant women, the elderly, infants, or those with HIV. L. monocytogenes can also cross the placental barrier in pregnant women, which can trigger a miscarriage.
“For immune-compromised consumers, it’s important to remember, that canned or cooked produce is better,” Deering said.
While commonly associated with ready-to-eat deli meat and hot dogs, outbreaks of listeriosis - the disease associated with the bacteria - have also been caused by contaminated celery, cantaloupe, sprouts and apples. A 2011 outbreak from cantaloupe was the second most deadly foodborne bacterial outbreak in U.S. history. The bacteria can only be killed by heat and the contaminated fruit that was minimally processed and consumed raw.
Symptoms of listeriosis may take as long as two months to appear, and by that time, most people don’t connect the illness to something they ate, Deering said.
After the 2016 recall of contaminated packaged salads, Deering, Haley Oliver, associate professor of food science, and Archana Shenoy, a graduate research assistant, began to investigate the persistence and internalization of L. monocytogenes in romaine lettuce, the fastest growing crop in the U.S. in terms of production, export and consumption.
Their research showed L. monocytogenes in romaine lettuce can persist up to 60 days or until the time of harvest. The bacteria could be found throughout the plant tissue, indicating yet another way foodborne pathogens can reach consumers, especially in ready-to-eat foods.
At the Purdue Center for Food Safety Engineering, researchers are working on detection technologies as they shift their focus to what can happen to the seed and seedlings before planting. They aim to find pre-harvest control strategies to prevent produce contamination, particularly as sanitizers can only treat produce externally.
“Continued education, training and research to minimize exposure of human foodborne pathogens in our soil, water, seeds, plants and produce have become my priority in research,” said Deering.
This article has been republished from materials provided by Purdue University. Note: material may have been edited for length and content. For further information, please contact the cited source.
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9th Edition of International Conference on Analytical Chemistry
Mar 19 - Mar 20, 2018