We've updated our Privacy Policy to make it clearer how we use your personal data. We use cookies to provide you with a better experience. You can read our Cookie Policy here.


New Markers for Forensic Body-fluid Identification

Listen with
Register for free to listen to this article
Thank you. Listen to this article using the player above.

Want to listen to this article for FREE?

Complete the form below to unlock access to ALL audio articles.

Read time: Less than a minute

Micro-RNAs (miRNAs) are a class of small noncoding RNA (ncRNA) molecules with a length of 18–24 nucleotides which play an essential regulative role for many cellular processes. Evidence suggests that the miRNome is a more precise and meaningful representation of a cell type and condition than the mRNA transcriptome. To identify miRNAs that are suitable for forensic body-fluid identification, a global screening by microarray analysis of c. 800 miRNAs of forensic blood and saliva samples was performed, and by bioinformatic processing, three differentially expressed candidate miRNAs for saliva and blood each were selected. The six candidates were then validated and confirmed via quantitative PCR. Herein, we present miRNA assays consisting of three differentially expressed miRNAs for the identification of blood (miR-126, miR-150, miR-451) and saliva (miR-200c, miR-203, miR-205), respectively. We conclude that miRNA extraction from forensic samples is possible and support a “proof of concept” that body-fluid identification by miRNA analysis may become a potent forensic technique.

Please note: The content above may have been edited to ensure it is in keeping with Technology Nteowrks' style and length guidelines. 


Courts, C., & Madea, B. (2011). Specific Micro-RNA signatures for the detection of saliva and blood in forensic body-fluid identification. Journal of Forensic Sciences, 56(6), 1464–1470. doi:10.1111/j.1556-4029.2011.01894.x