We've updated our Privacy Policy to make it clearer how we use your personal data.

We use cookies to provide you with a better experience. You can read our Cookie Policy here.


Protein Identified That Helps Plant Cope With Salt Stress

A square of cress on a grey background.
Credit: Luke Besley / Unsplash.
Listen with
Register for free to listen to this article
Thank you. Listen to this article using the player above.

Want to listen to this article for FREE?

Complete the form below to unlock access to ALL audio articles.

Read time: 1 minute
CONSTANS (CO), a well-known member of the B-box family, is the major component of the photoperiodically regulated flowering and circadian pathways in Arabidopsis (Arabidopsis thaliana). CO is expressed in various tissues, including seedling roots and young leaves. However, the roles and underlying mechanisms of CO in modulating physiological processes outside of flowering remain unclear 

In a study published in Plant Physiology, researchers from the Xishuangbanna Tropical Botanical Garden (XTBG) of the Chinese Academy of Sciences showed that CONSTANS protein negatively modulated salinity tolerance in Arabidopsis by physically associating with and antagonizing the ABSCISIC ACID-RESPONSIVE ELEMENT BINDING FACTOR (ABF) transcription factors under long-day conditions. 

The researchers first analyzed the spatial pattern of CO expression and detected its transcripts in young leaves and seedling roots. They observed that the CO transcription and proteins levels decreased in salt-stressed seedlings.  

Want more breaking news?

Subscribe to Technology Networks’ daily newsletter, delivering breaking science news straight to your inbox every day.

Subscribe for FREE
They then performed phenotypic analyses and found that CO negatively mediates salinity tolerance under long day (LD) conditions. The loss-of-function co mutant seedlings were more tolerant to salinity stress, whereas overexpression of CO resulted in plants with reduced tolerance to salinity stress. 

Genetic analyses revealed that the negative role of GIGANTEA (GI) in mediating salinity tolerance required a functional CO protein. Further investigations showed that CO physically interacts with the ABSCISIC ACID-RESPONSIVE ELEMENT BINDING FACTOR1 (ABF1), ABF2, ABF3, and ABF4, which belong to a large family of basic leucine zipper domains.

These ABFs were positively involved in the modulation of salinity tolerance. CO repressed the expression of several salinity-responsive genes and interfered with the transcriptional regulatory function of ABF3. 

"Our results suggest that CO antagonistically interacts with ABFs to inhibit salinity responses. This study provides a mechanistic understanding of how plant salinity tolerance is negatively regulated by CO functioning in conjunction with ABFs transcription factors," said HU Yanru of XTBG.

Reference: Du J, Zhu X, He K, et al. CONSTANS interacts with and antagonizes ABF transcription factors during salt stress under long-day conditions. Plant Physiology. 2023:kiad370. doi: 10.1093/plphys/kiad370

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.